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imaging procedures  (Miltenyi Biotec)


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    Miltenyi Biotec imaging procedures
    Imaging Procedures, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imaging+procedures/pm41482590-445-21-28?v=Miltenyi+Biotec
    Average 94 stars, based on 24 article reviews
    imaging procedures - by Bioz Stars, 2026-07
    94/100 stars

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    DZ-865B inhibits <t>proliferation</t> of DLBCL Cells In Vitro . ( A ). Soft agar colony formation assay of DLBCL cell line OCI-LY-1 after 21 days of treatment with various concentrations of DZ-865B, showing a dose-dependent decrease in colony formation. Scale bar: 10 μm. ( B ). Western blot analysis of OCI-LY-1 cells treated with BI-3802 and DZ-865B for 48 h, examining the expression levels of apoptosis and proliferation-related proteins, including Cleaved Caspase-3, Bax, Bcl-xL, and C-Myc, in response to increasing concentrations of each compound. ( C ). Western blot analysis of SU-DHL-4 cells treated with BI-3802 and DZ-865B for 48 h, assessing the expression of Cleaved Caspase-3, Bax, and Bcl-xL to evaluate apoptotic and anti-apoptotic protein modulation in a concentration-dependent manner. ( D , E ). EdU incorporation assay to assess DNA synthesis in OCI-LY-1 and SU-DHL-4 cell lines treated with 5 μm DZ-865B for 48 h. Immunofluorescence images show reduced EdU-positive cells after DZ-865B treatment, indicating decreased DNA synthesis and proliferation. Scale bar: 10 μm. ( F , G ). Quantitative analysis of EdU fluorescence intensity, measured as integrated density using ImageJ software. The control group did not receive DZ-865B treatment. Results indicate a significant reduction in DNA synthesis in DZ-865B-treated groups compared to control groups (** p < 0.01 vs. control)
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    Image Search Results


    DZ-865B inhibits proliferation of DLBCL Cells In Vitro . ( A ). Soft agar colony formation assay of DLBCL cell line OCI-LY-1 after 21 days of treatment with various concentrations of DZ-865B, showing a dose-dependent decrease in colony formation. Scale bar: 10 μm. ( B ). Western blot analysis of OCI-LY-1 cells treated with BI-3802 and DZ-865B for 48 h, examining the expression levels of apoptosis and proliferation-related proteins, including Cleaved Caspase-3, Bax, Bcl-xL, and C-Myc, in response to increasing concentrations of each compound. ( C ). Western blot analysis of SU-DHL-4 cells treated with BI-3802 and DZ-865B for 48 h, assessing the expression of Cleaved Caspase-3, Bax, and Bcl-xL to evaluate apoptotic and anti-apoptotic protein modulation in a concentration-dependent manner. ( D , E ). EdU incorporation assay to assess DNA synthesis in OCI-LY-1 and SU-DHL-4 cell lines treated with 5 μm DZ-865B for 48 h. Immunofluorescence images show reduced EdU-positive cells after DZ-865B treatment, indicating decreased DNA synthesis and proliferation. Scale bar: 10 μm. ( F , G ). Quantitative analysis of EdU fluorescence intensity, measured as integrated density using ImageJ software. The control group did not receive DZ-865B treatment. Results indicate a significant reduction in DNA synthesis in DZ-865B-treated groups compared to control groups (** p < 0.01 vs. control)

    Journal: Oncology Research

    Article Title: Novel Small Molecule DZ-865B Effectively Degrades BCL6, Promotes Apoptosis and Reduces Proliferation of Diffuse Large B-Cell Lymphoma Cells

    doi: 10.32604/or.2026.068695

    Figure Lengend Snippet: DZ-865B inhibits proliferation of DLBCL Cells In Vitro . ( A ). Soft agar colony formation assay of DLBCL cell line OCI-LY-1 after 21 days of treatment with various concentrations of DZ-865B, showing a dose-dependent decrease in colony formation. Scale bar: 10 μm. ( B ). Western blot analysis of OCI-LY-1 cells treated with BI-3802 and DZ-865B for 48 h, examining the expression levels of apoptosis and proliferation-related proteins, including Cleaved Caspase-3, Bax, Bcl-xL, and C-Myc, in response to increasing concentrations of each compound. ( C ). Western blot analysis of SU-DHL-4 cells treated with BI-3802 and DZ-865B for 48 h, assessing the expression of Cleaved Caspase-3, Bax, and Bcl-xL to evaluate apoptotic and anti-apoptotic protein modulation in a concentration-dependent manner. ( D , E ). EdU incorporation assay to assess DNA synthesis in OCI-LY-1 and SU-DHL-4 cell lines treated with 5 μm DZ-865B for 48 h. Immunofluorescence images show reduced EdU-positive cells after DZ-865B treatment, indicating decreased DNA synthesis and proliferation. Scale bar: 10 μm. ( F , G ). Quantitative analysis of EdU fluorescence intensity, measured as integrated density using ImageJ software. The control group did not receive DZ-865B treatment. Results indicate a significant reduction in DNA synthesis in DZ-865B-treated groups compared to control groups (** p < 0.01 vs. control)

    Article Snippet: EdU incorporation was detected using the EdU Method Cell Proliferation Imaging Analysis Kit (green fluorescence) (Cat. No. KTA2030, Abbkine, Wuhan, China) according to the manufacturer’s protocol.

    Techniques: In Vitro, Soft Agar Assay, Western Blot, Expressing, Concentration Assay, DNA Synthesis, Immunofluorescence, Fluorescence, Software, Control